With which objective should one begin when focusing the microscope?

By starting with an objective that offers lower magnification, such as 4x or 10x, it allows for easier locating and centering of the sample on the slide. Using a higher objective, such as 40x or 100x, may make it more difficult to find and focus on the sample initially. This is because the field of view becomes smaller and the image may appear blurry or dark if not properly focused. Therefore, it is important to start with a lower magnification objective before moving on to higher ones.